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Reference type: Journal
Authors: Çimen D, Bereli N, Andaç M, Denizli A
Article Title: Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract.
Publication date: 2018
Journal: Separation Science Plus
Volume: 1
Issue: (6)
Page numbers: 454-463.
DOI: 10.1002/sscp.201800039

Abstract: Concanavalin A, one of most studied lectins for the purification of glycoproteins and sugars, is selected as a model protein. In this study, a Concanavalin A imprinted poly(hydroxyethyl methacrylate) supermacroporous cryogel was prepared for the purification of Concanavalin from jack bean extract. N-Methacryloyl-l-histidine methyl ester with nickel(II) ions was used as the metal ion coordination complex. Concanavalin A imprinted cryogels were characterized by swelling degree, surface area, Fourier transform infrared spectroscopy, scanning electron microscopy and micro computed tomography measurements. Concanavalin A rebinding and desorption on Concanavalin A imprinted and non-imprinted cryogels were measured using a continuous system. Selective binding studies were carried out in the presence of Concanavalin B and bovine serum albumin. The selectivity studies were confirmed by fast protein liquid chromatography studies using jack bean extract from Canavalia ensiformis
Template and target information: protein, concanavalin B
Author keywords: concanavalin A, Cryogels, molecular imprinting


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