Abstract: A molecularly imprinted nanotubes membrane (AAO@MIP) was prepared using dopamine (DA) as template, porous anodic alumina (AAO) as reactor. The morphology of AAO@MIP was characterized by means of scanning electron microscopy (SEM). The specific adsorption performance of AAO@MIP to catecholamines (CLs) was investigated by high performance liquid chromatography (HPLC). Results of adsorption experiments revealed that the AAO@MIP has good extraction capacity and selectivity to dopamine, epinephrine and norepinephrine. A good linearity was achieved for CLs over the range of 0.50-300 μmol/L and the limit of detection (S/N=3) was found to be as low as 15.5, 12.6 and 22.5 ng/L for dopamine, epinephrine and norepinephrine, respectively. The maximum adsorption capacity of AAO@MIP to dopamine can reach 82.1 μmol/g. And the adsorption capacity is only reduced by 3.3% after six times adsorption-desorption. The proposed method was applied to the determination of CLs in human urine samples. The average recoveries ranged from 74.0% to 100.4% with the relative standard deviation (RSD) of 3.6%-6.8%. The developed method is rapid, selective and sensitive, and could be adapted to the analysis of CLs in urine samples.
Template and target information: dopamine, DA, catecholamines, CLs, epinephrine, norepinephrine
Author keywords: Porous anodic alumina (AAO), Molecularly imprinted nanotubes membrane, catecholamine, high performance liquid chromatography