MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Kellens E, Bové H, Vandenryt T, Lambrichts J, Dekens J, Drijkoningen S, D'Haen J, De Ceuninck W, Thoelen R, Junkers T, Haenen K, Ethirajan A
Article Title: Micro-patterned molecularly imprinted polymer structures on functionalized diamond-coated substrates for testosterone detection.
Publication date: 2018
Journal: Biosensors and Bioelectronics
Volume: 118
Page numbers: 58-65.
DOI: 10.1016/j.bios.2018.07.032
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566318305359

Abstract: Molecularly imprinted polymers (MIPs) can selectively bind target molecules and can therefore be advantageously used as a low-cost and robust alternative to replace fragile and expensive natural receptors. Yet, one major challenge in using MIPs for sensor development is the lack of simple and cost-effective techniques that allow firm fixation as well as controllable and consistent receptor material distribution on the sensor substrate. In this work, a convenient method is presented wherein microfluidic systems in conjunction with in situ photo-polymerization on functionalized diamond substrates are used. This novel strategy is simple, efficient, low-cost and less time consuming. Moreover, the approach ensures a tunable and consistent MIP material amount and distribution between different sensor substrates and thus a controllable active sensing surface. The obtained patterned MIP structures are successfully tested as a selective sensor platform to detect physiological concentrations of the hormone disruptor testosterone in buffer, urine and saliva using electrochemical impedance spectroscopy. The highest added testosterone concentration (500 nM) in buffer resulted in an impedance signal of 10.03 ± 0.19% and the lowest concentration (0.5 nM) led to a measurable signal of 1.8 ± 0.15% for the MIPs. With a detection limit of 0.5 nM, the MIP signals exhibited good linearity between a 0.5 nM and 20 nM concentration range. Apart from the excellent and selective recognition offered by these MIP structures, they are also stable during and after the dynamic sensor measurements. Additionally, the MIPs can be easily regenerated by a simple washing procedure and are successfully tested for their reusability
Template and target information: testosterone
Author keywords: Microfluidics, Molecularly imprinted polymers, Biosensors and Body fluids

  Shirt featuring the name Ward spelled out in symbols of the chemical elements  Lab Hero Periodic tablemug  HE-MAN polymer chemistry shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner