MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Arifuzzaman M, Zhao Y
Article Title: Artificial Zinc Enzymes with Fine-Tuned Active Sites for Highly Selective Hydrolysis of Activated Esters.
Publication date: 2018
Journal: ACS Catalysis
Page numbers: 8154-8161.
DOI: 10.1021/acscatal.8b02292

Abstract: Zinc enzymes are ubiquitous in nature and are frequently used to catalyze the hydrolysis of carboxylic acid esters, phosphate esters, and amides. Although many models and mimics of zinc enzymes have been reported, it remains difficult to construct active sites with accurately positioned catalytic groups and tunable substrate selectivity. By imprinting a substrate-like amino template coordinated to a polymerizable zinc complex inside cross-linked micelles, we prepared water-soluble nanoparticles with well-defined active sites. The position of the zinc ion could be tuned systematically with respect to the ester bond to be cleaved in the substrate (p-nitrophenyl esters), as well as the rigidity of the active site. Our imprinted zinc catalysts were able to distinguish substrates that differed by the position of a single methyl group, chain length of the acyl chain, and substitution of the phenyl ring. The turnover number (>460 at pH 7) was 1 order of magnitude higher than those previously reported for artificial zinc enzymes in the literature
Template and target information: catalyst, transition state analogue, TSA
Author keywords: active site, artificial enzyme, biomimetic catalysis, hydrolysis, Michaelis-Menten, molecular imprinting, selectivity

  Periodic table Cream Cakes - the chemical formula for naughty but nice apron  Periodic table Bag bag  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner