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Reference type: Journal
Authors: Kaur R, Kaur R, Rani S, Malik AK
Article Title: Molecularly Imprinted Polymers-Solid Phase Extraction Coupled With HPLC-FLD for the Analysis of Four Fluoroquinolones in Honey and Urine.
Publication date: 2018
Journal: Journal of Pharmaceutical, Chemical and Biological Sciences
Volume: 6
Issue: (2)
Page numbers: 60-69.
Alternative URL: https://www.jpcbs.info/2018_6_2_07_Kaur.pdf

Abstract: A new, fast and sensitive method using molecularly imprinted polymers-solid phase extraction (MIPs-SPE) followed by high pressure liquid chromatography with fluorescence detection (HPLC-FLD) has been developed for the separation and determination of four fluoroquinolones (FQs) from honey and urine. Detection has been done by fluorescence measurement with excitation wavelength 300 nm and emission wavelength 450 nm. The best separation was achieved in mobile phase consisting of water, acetonitrile, and 1% formic acid (21:9:70 v/v). Sample preconcentration and sample clean-up was done by molecularly imprinted polymer solid-phase extraction and the developed method has been applied to spiked samples of honey and urine for extraction of four fluoroquinolones namely norfloxacin (NOR), ofloxacin (OFLO), lomefloxacin (LOME) and danofloxacin (DANO). Detection limits are in the low range of 0.02 - 0.287 ng /mL.
Template and target information: norfloxacin, NOR, ofloxacin, OFLO, lomefloxacin, LOME, danofloxacin, DANO
Author keywords: HPLC-FLD, MIPs-SPE, Fluroquinolones


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