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Abstract: In this study, we report label-free and selective histamine detection using a molecularly imprinted polymer (MIP)-coated gate field-effect transistor (FET). First, we designed the histamine-selective MIP; methacrylic acid derivatives were chosen as a functional monomer to induce the change in molecular charges based on the histamine/monomer complex in the prepolymer solution, because histamine contains several hydrogen bond acceptors such as amino groups; moreover, the crosslinker/monomer ratio in the polymer was controlled to strengthen the structure of the polymer matrix and to enhance the selectivity of the MIP to histamine. As a result, the histamine-templated MIP (HMIP)-coated FET showed a good electrical response to histamine at a concentration of 100 μM and above, enabling the detection of histamine secreted from mast cells based on allergic responses. Moreover, more selective detection to histamine was realized by using an HMIP with higher crosslink density, as shown by comparing the electrical response upon adding L-histidine to an HMIP-FET, which has a similar chemical structure to histamine. The enhancement of selectivity to histamine was clarified on the basis of the binding constant estimated by potentiometric Langmuir isotherm analysis
Template and target information: histamine
Author keywords: field-effect transistor (FET), histamine, molecularly imprinted polymer (MIP)