MIPdatabase
Authors: Bartold K, Pietrzyk-Le A, Golebiewska K, Lisowski W, Cauteruccio S, Licandro E, D'Souza F, Kutner W
Article Title: Oligonucleotide Determination via Peptide Nucleic Acid Macromolecular Imprinting in an Electropolymerized CG-Rich Artificial Oligomer Analogue.
Publication date: 2018
Journal: ACS Applied Materials & Interfaces
Volume: 10
Issue: (33)
Page numbers: 27562-27569.
DOI: 10.1021/acsami.8b09296
Abstract: We devised and fabricated a chemosensor for determination of the genetically relevant 5'-GCGGCGGC-3' (G = guanine; C = cytosine) oligonucleotide. For that, we simultaneously electrosynthesized and electrode-immobilized a sequence-defined octakis(2,2'-bithien-5-yl) DNA hybridizing probe using both a "macromolecular imprinting in polymer strategy" and a sequence-programmable peptide nucleic acid (PNA) template. With electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) transductions under stagnant-solution and flow injection analysis (FIA) conditions, respectively, we determined the above oligonucleotide with 200-pM EIS limit of detection. With its EIS-determined apparent imprinting factor of ~4.0, the chemosensor was discriminative to both mismatched oligonucleotides and Dulbecco's modified Eagle's medium sample interferences
Template and target information: peptide nucleic acid, PNA, DNA, 5'-GCGGCGGC-3', oligonucleotide
Author keywords: CG-rich DNA analogue, DNA sensor, electrochemical impedance spectroscopy, hybridization, isothermal titration calorimetry, macromolecularly imprinted polymer, peptide nucleic acid
Join the Society for Molecular Imprinting
New items RSS feed
View latest updatesSign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.
Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
