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Reference type: Journal
Authors: Beloglazova N, Lenain P, Tessier M, Goryacheva I, Hens Z, De Saeger S
Article Title: Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone.
Publication date: 2019
Journal: Talanta
Volume: 192
Page numbers: 169-174.
DOI: 10.1016/j.talanta.2018.09.042
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0039914018309500

Abstract: A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO2 labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 μg kg-1 in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results
Template and target information: bioimprinting, deoxynivalenol, zearalenone
Author keywords: bioimprinting, Quantum dots, Silica coated quantum dot, immunoassay, zearalenone, deoxynivalenol

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