MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Beloglazova N, Lenain P, Tessier M, Goryacheva I, Hens Z, De Saeger S
Article Title: Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone.
Publication date: 2019
Journal: Talanta
Volume: 192
Page numbers: 169-174.
DOI: 10.1016/j.talanta.2018.09.042
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0039914018309500

Abstract: A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO2 labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 μg kg-1 in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results
Template and target information: bioimprinting, deoxynivalenol, zearalenone
Author keywords: bioimprinting, Quantum dots, Silica coated quantum dot, immunoassay, zearalenone, deoxynivalenol


  Periodic table Teacher shirt  Mug featuring the name Sarah spelled out in the single letter amino acid code  We need to talk about Kelvin shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner