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Abstract: The authors describe a fluorescent probe for sensitive and selective determination of quercetin, an indicator for the freshness of drinks. The probe consists of silica ball encapsulated graphitic carbon nitride (g-C3N4) modified with a molecularly imprinted polymer (MIP). It was synthesized via reverse microemulsion. The resulting MIP@g-C3N4 nanocomposite was characterized by fluorescence spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray powder diffraction. Quercetin quenches the fluorescence of the MIP@g-C3N4 probe. The effect was used to quantify quercetin in grape juice, tea juice, black tea, and red wine by fluorometry (λexc = 350 nm, λem = 460 nm). Response is linear in the 10-1000 ng mL-1 quercetin concentration range. The detection limit is 2.5 ng mL-1, recoveries range between 90.7 and 94.1%, and relative standard deviations are between 2.1 and 5.5%
Template and target information: quercetin
Author keywords: Soft drinks, Graphite carbon nitride, molecular imprinting, π-Interaction, Probe, fluorescence, flavonoids, Reverse microemulsion