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Abstract: In this study, a molecularly imprinted polymer (MIP)-fluorescent probe based on hydrophobic CdSe/ZnS quantum dots (CdSe/ZnS@MIP) was designed and synthesized by bulk polymerization for detecting methamidophos (MAP). The CdSe/ZnS@MIP was characterized by Fourier-transform infrared spectroscopy, thermo-gravimetric analysis and adsorption properties, and it exhibited good adsorption capacity and fluorescent stability, and excellent selectivity. Under optimal conditions, the fluorescence showed a good linear decrease in the range of 3.50 x 10-7 - 0.71 x 10-3 mol/L with a correlation coefficient of 0.995. The limit of detection of the fluorescent probe was 9.16 x 10-8 mol/L, and the relative standard deviation of the method for nine replicate analyses of 4.0 x 10-6 mol/L MAP was 4.1%. Apple and pear samples spiked with MAP at three levels were extracted and determined by the presented method with good recoveries (89.7%-94.9%). The accuracy of the method was verified by gas chromatography for the detection of kidney beans, leek and cucumber samples, and no significant difference was observed for the results of two methods
Template and target information: methamidophos, MAP
Author keywords: Fluorescent probe, Methamidophos, molecular imprinting, quantum dot