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Reference type: Journal
Authors: Sanbe H, Hosoya K, Haginaka J
Article Title: Preparation of uniformly sized molecularly imprinted polymers for phenolic compounds and their application to the assay of bisphenol A in river water.
Publication date: 2003
Journal: Analytical Sciences
Volume: 19
Issue: (5)
Page numbers: 715-719.
DOI: 10.2116/analsci.19.715
Alternative URL: http://www.jstage.jst.go.jp/article/analsci/19/5/715/_pdf

Abstract: Uniformly sized molecularly imprinted polymers, which can recognize bisphenol A (BPA), have been prepared by a multi-step swelling and polymerization method using BPA or a structurally related analogue of BPA [p-t-octylphenol (OP) or p-t- butylphenol (BP)] as the template molecule, 4-vinylpyridine as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker. The BP-imprinted polymer showed higher molecular recognition ability for BPA than the OP-imprinted polymer. The BPA- and BP-imprinted polymers were applied for the assay of a trace amount of BPA in river water using column- switching HPLC with fluorescence detection: A BPA-imprinted polymer was used for removal of BPA from the pretreatment eluent as the trap column, and a BP-imprinted polymer was used for selective pretreatment and enrichment of BPA in river water as the pretreatment column. The calibration graph, constructed from peak area data plotted versus BPA concentration, was linear with a correlation coefficient of >0.999 in the concentration ranges of 25 - 1000 ppt. The limit of quantitation was 25 ppt with a 5-ml injection. The column- switching HPLC system was successfully applied for the assay of BPA in river water


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