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Reference type: Journal
Authors: Cardoso AR, Marques AC, Santos L, Carvalho AF, Costa FM, Martins R, Sales MGF, Fortunato E
Article Title: Molecularly-imprinted chloramphenicol sensor with laser-induced graphene electrodes.
Publication date: 2019
Journal: Biosensors and Bioelectronics
Volume: 124-125
Page numbers: 167-175.
DOI: 10.1016/j.bios.2018.10.015
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566318308285

Abstract: Graphene has emerged as a novel material with enhanced electrical and structural properties that can be used for a multitude of applications from super-capacitors to biosensors. In this context, an ultra-sensitive biosensor was developed using a low-cost, simple and mask-free method based on laser-induced graphene technique for electrodes patterning. The graphene was produced on a polyimide substrate, showing a porous multi-layer structure with a resistivity of 102.4 7.3 [Omega]/square. The biosensor was designed as a 3-electrode system. Auxiliary and working electrodes were made of graphene by laser patterning and the reference electrode was handmade by casting a silver ink. A molecularly-imprinted polymer (MIP) was produced at the working electrode by direct electropolymerization of eriochrome black T (EBT). As proof-of-concept, the MIP film was tailored for chloramphenicol (CAP), a common contaminant in aquaculture. The resulting device was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy readings against a redox standard probe. The limit of detection (LOD) was 0.62 nM and the linear response ranged from 1 nM to 10 mM. These analytical features were better than those produced by assembling the same biorecognition element on commercial graphene- and carbon-based screen-printed electrodes. Overall, the simplicity and quickness of the laser-induced graphene technique, along with the better analytical features obtained with the graphene-based electrodes, shows the potential to become a commercial approach for on-site sensing
Template and target information: chloramphenicol, CAP
Author keywords: Laser irradiation, On-site graphene production, Three-electrode system, molecularly-imprinted polymer, chloramphenicol

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