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Reference type: Journal
Authors: Wang L, Song X, Wang Q, Feng XQ, Xu R, Hang XY
Article Title: Determination of ciprofloxacin in milk by new magnetic molecular imprinting- high performance liquid chromatography.
Publication date: 2018
Journal: Journal of Food Safety and Quality
Volume: 9
Issue: (15)
Page numbers: 3999-4005.
Alternative URL: http://www.chinafoodj.com/ch/reader/view_abstract.aspx?file_no=20180507002&flag=1

Abstract: Objective: To establish a method for determination of the content of ciprofloxacin in milk by new magnetic molecular imprinting-high performance liquid chromatography. Methods: The new magnetic molecular imprinting material was synthesized with CoFe2O4--graphene as the carrier, dopamine as the functional monomer and ciprofloxacin as the template molecule. After the milk sample was extracted with acetonitrile, the new magnetic molecular imprinting material was used to adsorb the ciprofloxacin. The solution was eluted by methanol-acetic acid (9:1, V:V), filtered through membrane, and quantitative determination was carried out by high performance liquid chromatograph. Results: The new magnetic molecular imprinting material could reach the adsorption equilibrium rapidly within 15 minutes and had good identification. Under the best experimental conditions, ciprofloxacin showed a good relationship between peak areas and concentrations in the concentration range of 0.5-20 μg/L (r2=0.9986) and the limit of detection was 0.09 μg/L. The recoveries were 91%-105.1% with relative standard deviation of 1.9%-4.7%. Conclusion: This method is simple to operate and can be used for quantitative detection of ciprofloxacin in milk
Template and target information: ciprofloxacin
Author keywords: CoFe2O4-graphene, Magnetic molecular imprinted polymers, Ciprofloxacin, high performance liquid chromatography


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