MIPdatabase
Authors: Wu ZZ, He DY, Cui B, Jin ZY
Article Title: Ultrasensitive detection of microcystin-LR with gold immunochromatographic assay assisted by a molecular imprinting technique.
Publication date: 2019
Journal: Food Chemistry
Volume: 283
Page numbers: 517-521.
DOI: 10.1016/j.foodchem.2019.01.064
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0308814619301323
Abstract: A simple and highly sensitive lateral flow immunochromatographic assay (LFICA) towards microcystin-LR (MC-LR) was proposed in this study. Molecular imprinting technique was combined with enzyme assisted colorimetric method to substantially enhance the sensitivity of traditional LFICA. The target, i.e., MC-LR molecular, was first separated from the complex matrix by molecularly-imprinted polymers (MIPs). Then, the obtained MC-LR was detected by LFICA based on the blue color generated by an enzyme–substrate reaction. Using the proposed assay, under optimal conditions, the determination of MC-LR was doable in a wide linear range from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.04 ng/mL. In addition, the reliability of the developed method was validated by determining MC-LR contents in real samples. The overall results suggested that the neoteric LFICA method possess great potential in sensitive detection of MC-LR
Template and target information: microcystin-LR, MC-LR
Author keywords: microcystin-LR, Rapid analysis, gold nanoparticles, Lateral flow, molecular imprinting
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