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Abstract: The fabrication of molecularly imprinted nanoparticles (MIP-NPs) specific for myoglobin by using thiol-ene photopolymerization in miniemulsion was described. Allyl derivatives of phenylalanine as a functional monomer was synthesized and copolymerized with acrylic monomers via miniemulsion polymerization to produce NIP-NPs with approximately 74 nm number average particle diameter. FTIR and 1H-NMR analysis confirmed the synthesis of functional monomer. MIP-NPs were prepared in the existence of myoglobin as a template protein. Morphological investigations exhibited that the particle size of the MIP-NPs, increased compared to the corresponding NIPs and the mean particle diameter by number was measured as 141 nm with narrow distribution. NIP-NPs that were polymerized without myoglobin were found to have less affinity to the target protein. In addition, the rebinding ability of MIP-NPs was much bigger than that of the corresponding NIPs. ELISA results showed that MIPs interact particularly with the myoglobin and show little affinity for BSA in competitive binding experiments.Highlights: Allyl N,N-diallyl phenylalaninate was synthesized as a functional monomer;Imprinted nanoparticles were prepared by using thiol-ene photopolymerization in miniemulsion;The nanoparticles were 141 nm with narrow size distribution;The imprinted nanoparticles showed selectivity toward myoglobin
Template and target information: protein, myoglobin
Author keywords: molecularly imprinted polymer, thiol-ene polymerization, photopolymerization, myoglobin, plastic antibody