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Abstract: In the last years, the imprinting technology has raised attention and different polymers with artificial recognition sites towards a target molecule have been developed. In this article, a polymerization-free method -which only requires the dissolution of nylon-6 polymer and its subsequent incubation with the template molecule- has been employed for the preparation of a molecularly imprinted polymer directly immobilized on filter paper, thus increasing the versatility and manageability of the new material. The molecularly imprinted paper-based analytical device (MIP PAD) has been coupled to a fluorimeter via a custom-built platform, which enables direct measurement of the fluorescence at the surface of the MIP PAD. Extraction efficiency of the MIP PAD was evaluated using quinine as model analyte, measuring the fluorescence directly at the MIP PAD surface after incubation on aqueous standards of quinine and soda drink, showing that the performance of the imprinted material was superior to that of the non-imprinted polymer. In addition, the selectivity of the MIP PAD versus analogous organic fluorescent molecules has been further evaluated using norfloxacin, observing that the molecular imprinting results in selective extraction of quinine. The performance of the method has been evaluated for quantitative analysis, achieving limits of detection (LOD) and quantification (LOQ) for aqueous standards of 0.37 and 1.24 mg L-1, respectively, while in the case of spiked soda drink the LOD and LOQ were 0.63 and 2.11 mg L-1, respectively. The reproducibility of the measurements was also evaluated, observing values within range 2.30 and 9.07% measured as relative standard deviation
Template and target information: quinine
Author keywords: molecularly imprinted polymer, nylon-6, microextraction, Cellulose paper, quinine, fluorescence, Paper-based analytical device