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Abstract: Herein, a near-infrared (NIR) light-responsive imprinting biosensor with excellent conductivity and tensibility was designed by incorporation graphene oxide/polyaniline (GO/PANI) with thermo-responsive poly(N-isopropylacrylamide) hydrogel (PNiPAAm) on a glassy carbon electrode (GCE). In this design, polyaniline (PANI) nanofiber modifies with graphene oxide (GO) acts as a physically cross-linker, providing hydrogen bond interaction with amide groups in the PNiPAAm. It is functioned to enhance the strength of imprinted hydrogel networks. Thus, the imprinted hydrogel biosensor incorporation of GO/PANI nanocomposite exhibited excellent mechanical performance and NIR light-responsive characteristic. As a NIR radiation trigger gate, the obtained biosensor not only has a function of IR-based cleaning toward bovine serum albumin (BSA) in the absence of elution solution, but also a function of proteins releasing and uptaking, basing on reversibly conformational changes with 808 nm NIR light. Simultaneously, the responsive processes were electronically monitored by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) and verified by scanning electron microscopy (SEM). Moreover, the proposed GO/PANI nanocomposite imprinted biosensor exhibited excellent characteristics along with high conductivity and tensibility, long-term stability, and wide linear range, performing great promising for preparing remote NIR light-driven extraction protein smart devices
Template and target information: protein, bovine serum albumin, BSA
Author keywords: Near-infrared light-responsive materials, Electrochemical imprinting biosensor, Bovine serum albumin, Nanocomposites conducting hydrogel