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Reference type: Journal
Authors: Guo XX, Yao S, Li H, Shi XN, Pang B, Jin J, Su ZY, Zhang HW, Zhao C, Wang J
Article Title: Multi-functional magnetic molecular imprinting probe for visual detection of IgY antibodies.
Publication date: 2021
Journal: Microchimica Acta
Volume: 188
Issue: (11)
Article Number: 378.
DOI: 10.1007/s00604-021-05040-4

Abstract: Egg yolk antibodies (IgY) are an alternative to mammalian antibodies, which have been successfully applied in treatment of disease, as immunochemical reagents and as food additives. A fast, accurate, and easy-to-operate detection method for IgY antibodies is needed. Herein, we developed a rapid and cost-effective colorimetric assay for the ultrasensitive detection of anti-S. aureus IgY antibodies using multi-functional magnetic molecularly imprinted polymers (MMIPs). The prepared MMIPs can recognize, adsorb, and separate the analyte from the matrix efficiently, and oxidize TMB to generate a colorimetric signal within ~ 60 min. As low as 0.013 mg mL-1 was able to be detected by using this method. The visual detection limit reached 0.02 mg mL-1. By testing the IgY in milk samples, the feasibility was verified
Template and target information: protein, egg yolk antibodies, IgY
Author keywords: Egg yolk antibodies, Magnetic molecularly imprinted polymers, Colorimetry, Food additives

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