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Abstract: Quercetin, a plant flavonoid with potent antioxidant action, has observed numerous health benefits. However, it is difficult to determine quercetin in natural products because of its complexity. Besides, molecular imprinted polymer (MIP) exhibits high selective, affinity and stability. To simplify the procedure of sample treatment, the quercetin imprinted polymer was synthesized by precipitation polymerization method, then applied as sorbent for selective solid-phase extraction. The aim of our study was to determine the suitable conditions of polymer polymerization reaction for high selective absorption of quercetin imprinted polymer in nano-size, directed to apply as sorbent for selective solid-phase extraction. MIP was synthesized by mixing Acrylic acid (AA), ethylene glycol dimethacrylate (EGDMA), 2,2'-azobisisobutyronitril (AIBN), quercetin in a large amount of porogen at 70℃ in 8 hours. The prepared quercetin-MIP was employed as the sorbent for solid extraction phase column (Quercetin-MISPE). The results of IR, UV-Vis confirmed the presence of template molecules only in MIP. The MIP targeting quercetin could absorb and rebind quercetin. The productivity of this method was 10%. The selective absorption of quercetin-MIP was demonstrated by comparing quercetin with rutine, kaempferol and isorhamnetin via HPLC analysis. We have successfully synthesized the molecular imprinted polymer for quercetin by using precipitation polymerization method, prepared and employed the quercetin-MIP as the sorbent for solid extraction phase column and investigate its characteristics. Quercetin absorption of MIP is 9.7% higher (2.85x) compared to rutine of 3.4%, while there is no absorption in case of NIP (0%) and MIP with kaempferol (0%) and isorhamnetin (0 %).
Template and target information: quercetin
Author keywords: quercetin, Quercetin imprinted polymer, Precipitation polymerization method, Selective solid-phase extraction