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Reference type: Journal
Authors: Wubulikasimu M, Muhammad T, Imerhasan M, Hudaberdi N, Yang WW, Zhao JZ, Peng XJ
Article Title: Synthesis of fluorescent drug molecules for competitive binding assay based on molecularly imprinted polymers.
Publication date: 2019
Journal: RSC Advances
Volume: 9
Issue: (12)
Page numbers: 6779-6784.
DOI: 10.1039/C9RA00422J

Abstract: Fluorescent immunosorbent assay (FIA) is very promising for sensitive and selective analysis in bio-medical applications. Here, we proposed an assay, using fluorescent engineering of analytes and the corresponding molecularly imprinted polymers (MIPs) as a plastic antibody. Three drug molecules (metronidazole, zidovudine and lamivudine) were condensed with 9-aminoacridine, using succinic anhydride as a spacer. The target products were characterized with 1H-NMR, IR and mass spectrometry. UV-vis absorption and fluorescent properties of the fluorophore-labeled drug molecules were investigated. Feasibility of the fluorescent biomimetic immunosorbent assay based on MIPs was demonstrated in the solution. This work will provide sound foundation for the future application in real sample
Template and target information: metronidazole, zidovudine, lamivudine

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