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Abstract: In this study, creatinine was detected using an artificial receptor based on the Molecularly Imprinted Polymer (MIP) method. Our fabrication method results in an MIP polyacrylamide hydrogel (PAAm) in the form of 1 mm-thick sheets with sufficient strength to be easily molded into a variety of shapes. The monomer-crosslinker ratio was optimized in order to achieve a semi-rigid hydrogel and to resist damage of hydrogen bonds that can occur during washing and rebinding steps. For a 0.3 mM creatinine rebinding solution, an imprinting factor of 2.54 was measured. To test selectivity, a 0.3 mM N-hydroxysuccinimide analogue rebinding solution was used. The recognition of the molecularly imprinted polyacrylamide for creatinine in the presence of this analogue was measured to give a binding selectivity ratio of 3.55. These results demonstrate that the PAAm hydrogel can be effectively used as an imprinting platform in aqueous solution for preserving bioactivity of creatinine
Template and target information: creatinine
Author keywords: hydrogel, creatinine, molecularly imprinted polymer, polyacrylamide, Absorbance, receptor