MIPdatabase
Authors: Lyu HX, Sun HR, Zhu YM, Wang J, Xie ZH, Li JX
Article Title: A double-recognized aptamer-molecularly imprinted monolithic column for high-specificity recognition of ochratoxin A.
Publication date: 2020
Journal: Analytica Chimica Acta
Volume: 1103
Page numbers: 97-105.
DOI: 10.1016/j.aca.2019.12.052
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0003267019315223
Abstract: In this study, a double-recognized aptamer-molecularly imprinted monolithic column (Apt-MIP monolithic column) was prepared by introducing both aptamer and MIP to reduce non-specific adsorption. Its preparation parameters such as the time of photo-initiation, the dosage of photo-initiator and the concentration of aptamer were investigated in detail. The recovery ratios of ochratoxin A (OTA) to ochratoxin B (OTB) on Apt-MIP monolithic column, Apt monolithic column and MIP monolithic column were 116.1, 40.8 and 69, respectively. Even if the concentration of OTB was 10 times that of OTA, the recovery of OTB was only about 2.9%. Applied to beer samples, the prepared Apt-MIP monolithic column drastically resisted background adsorption and the high-specificity recognition for OTA was obtained with the recoveries of 95.5-105.9%. This work provided a simple and effective method to selectively identify OTA from complex samples
Template and target information: ochratoxin A, OTA
Author keywords: aptamer, molecularly imprinted polymer, ochratoxin A, monolithic column, Double recognition
Join the Society for Molecular Imprinting
New items RSS feed
View latest updatesSign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.
Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
