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Abstract: This study proposed the developed of a molecularly imprinted polymer for the extraction and determination of condensed tannins from the barks of Red Angico (Anadenanthera macrocarpa), Jabuticaba (Myrciaria jabuticaba) and Umbu (Spondias tuberosa). The polymer was synthesized using the condensed tannin extracted from the Red Angico bark as the template molecule, as well as, catechin standard solution. Selectivity and characterization tests for the molecularly imprinted polymers and a non-imprinted polymer were performed. The polymers were employed as extraction phase for the solid-phase extraction of condensed tannins from the studied samples. A higher imprinting coefficient was obtained for MIP synthesized from catechin standard solution as template. The intrinsic solid-phase extraction variables were evaluated and optimized. The developed methodology showed inter- and intra-day precisions of 6.7-10.1 and 4.6-8.4, respectively, and recovery values ranging from 101.9 to 105.5. The obtained limits of detection and quantification were 10 mg L-1 and 40 mg L-1, respectively. It is important to highlight that the developed methodology here was applied to common waste and tailings from Brazilian food industry. The results indicate that the polymers were capable to extract tannins from the evaluated samples, reducing method cost and time
Template and target information: catechin, condensed tannins
Author keywords: Condensed tannin, molecularly imprinted polymer, natural products, Catechin