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Reference type: Journal
Authors: Siqueira Silva M, Moreira Tavares AP, Leomil Coelho LF, Morganti Ferreira Dias LE, Chura-Chambi RM, Guimar+˙es da Fonseca F, Ferreira Sales MG, Costa Figueiredo E
Article Title: Rational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein.
Publication date: 2021
Journal: Biosensors and Bioelectronics
Volume: 191
Article Number: 113419.
DOI: 10.1016/j.bios.2021.113419
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0956566321004565

Abstract: Rational selection of predicted peptides to be employed as templates in molecular imprinting was carried out for the heat-denatured non-structural protein 1 (NS1) of dengue virus (DENV). Conservation analysis among 301 sequences of Brazilian isolates of DENV and zika virus (ZIKV) NS1 was carried out by UniProtKB, and peptide selection was based on in silico data of the conservational, structural and immunogenic properties of the sequences. The selected peptide (from dengue 1 NS1) was synthesized and employed as a template in the electropolymerization of polyaminophenol-imprinted films on the surface of carbon screen-printed electrodes. Heat denaturation of the protein was carried out prior to analysis, in order to expose its internal hidden epitopes. After removal of the template, the molecularly imprinted cavities were able to rebind to the whole denatured protein as determined by electrochemical impedance spectroscopy. This label-free sensor was efficient to distinguish the NS1 of DENV from the NS1 of ZIKV. Additionally, the sensor was also selective for dengue NS1, in comparison with human serum immunoglobulin G and human serum albumin. Additionally, the device was able to detect the DENV NS1 at concentrations from 50 to 200 μg L-1 (RSD below 5.04%, r = 0.9678) in diluted human serum samples. The calculated LOD and LOQ were, respectively, 29.3 and 88.7 μg L-1 and each sensor could be used for six sequential cycles with the same performance
Template and target information: virus, peptide, epitope, non-structural protein 1, NS1, dengue virus, DENV, zika virus, ZIKV
Author keywords: DENV, NS1, Molecularly imprinted polymers, sensor, Epitope, label-free


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