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Abstract: We present a novel amperometric dual-imprinted sensor for simultaneous determination of creatinine (Cre) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in human urine and serum. The sensor used multiple-pulse amperometric detection in flow injection analysis (MPA-FIA). Copper oxide nanoparticles were coated with the Cre molecularly-imprinted polymer (CuO@MIP), using methacrylic acid as the functional monomer and N, N'-(1,2-dihydroxyethylene) bis-acrylamide as cross-linker. For 8-OHdG sensing, we embedded platinum nanoparticles in reduced graphene oxide and then coated it with guanosine poly-dopamine MIP (PtNPs-rGO@MIP). A carbon-paste electrode (CPE) was then formed containing both nanocomposites to give the dual MIP sensor (CuO@MIP and PtNPs-rGO@MIP/CPE). We developed a dual-potential waveform as a function of time, with Edet.1 (+0.4 V/150 ms) to determine Cre selectively and Edet.2 (+0.6 V/250 ms) to analyze both compounds simultaneously (Cre and 8-OHdG). Subtracting the two signals at 0.6 V and 0.4 V (using a correction factor), respectively, from each other allowed for quantifying 8-OHdG without interference from Cre. The MIP sensor has a linear range of 0.5-150 μM for creatinine and 0.005-50 μM for 8-OHdG, with limits of detection in nano-molar level. The proposed method is successfully applied for the simultaneous determination of Cre and 8-OHdG in urine and serum samples
Template and target information: dual template, creatinine, Cre, 8-hydroxy-2'-deoxyguanosine, 8-OHdG
Author keywords: MIP sensor, serum, urine, Simultaneous analysis, FIA