Alternatively for mipdatabase quick searches go here

Custom Search

Abstract: Fumonisin B1 (FB1) is a carcinogenic mycotoxin contaminating food and animal feed. Although the sensitivity of chromatography and immunoassays is high for FB1 determination, these techniques are laborious, costly, and challenging in making them portable. Herein, a highly sensitive and selective electrochemical sensor using molecularly imprinted polymer nanoparticles (nanoMIPs) for FB1 recognition was devised and fabricated. NanoMIPs were prepared by free-radical polymerisation using the solid-phase synthesis and characterised by DLS, SPR, SEM, AFM, and FTIR spectroscopy. The chemosensor fabrication was prepared in two steps. First, a film of the conducting polypyrrole-(zinc porphyrin) composite was deposited on a Pt electrode by electropolymerisation. Then, nanoMIPs were covalently attached to this film. Both electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) served for analytical signal transduction. The respective chemosensor sensitivity was 0.442 kΩ /pM (R2 = 0.98) and 0.281 μA/pM (R2 = 0.96) in the concentration range of 1 fM to 10 pM FB1. The limit of detection was impressively low, equalling to 0.03 and 0.7 fM, respectively, while the DPV determined imprinting factor was appreciably high 6.28. This electrochemical sensor revealed no cross-reactivity to other mycotoxins. The FB1 recovery in the FB1 spiked maize was high (96-102 %). Thus, the chemosensor proved useful as an effective alternative tool for FB1 determination in maize
Template and target information: fumonisin B1
Author keywords: NanoMIPs, chemosensor, Electrochemical sensor, EIS, DPV, Fumonisin B1