MIPdatabase
Authors: Smolinska-Kempisty K, Guerreiro A, Czulak J, Piletsky S
Article Title: Negative selection of MIPs to create high specificity ligands for glycated haemoglobin.
Publication date: 2019
Journal: Sensors and Actuators B: Chemical
Volume: 301
Article Number: 126967.
DOI: 10.1016/j.snb.2019.126967
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0925400519311669
Abstract: The solid-phase imprinting method was used to prepare particles imprinted against glycated haemoglobin by using the whole protein as a template. Obtained MIPs were then treated through a negative selection process to obtain high affinity MIPs able to recognize glycated haemoglobin but without any cross-reactivity towards non-glycated haemoglobin. Both, bulk and selected particles were tested in competitive pseudo-ELISA assays with haemoglobin and glycated haemoglobin as free targets. In both cases, a linear response was observed from 10-3 nM to 1 nM. After negative selection, MIPs showed no response to non-glycated haemoglobin together with an improved of the linear range of the assay. It also allowed quantification of glycated haemoglobin in human serum with recoveries above 95%
Template and target information: glycated haemoglobin, protein
Author keywords: Molecularly imprinted polymers, Differential process, ELISA assays, Non-Glycated haemoglobin
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