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Abstract: Polyphenols are bioactive substances of vegetal origin with a significant impact on human health. The assessment of polyphenol intake and excretion is therefore important. In this work, a new electrochemical approach based on molecularly imprinted polymer extraction and preconcentration, combined with a disposable carbon screen-printed sensor and adsorptive transfer differential pulse voltammetry detection has been proposed for quantifying of 4-hydroxyphenylacetic acid (4-HPA), which is a biomarker of flavan-3-ols intake, and other phenolic acids. The simple experimental performance has allowed the rapid data collection with relevant information about the profile of catabolites extracted. The method was validated over a concentration range of 10-200 mg L-1, R2 > 0.999. In the optimized conditions, the recovery value was 94% with RSD 8%. The limits of detection and quantification were 2.38 mg L-1 and 7.21 mg L-1, respectively. The method was validated by means of a chromatographic method, being the differences between the values of the 4-HPA concentrations obtained by both methods under 1%. The proposed method showed high recoveries, low detection limit, and good accuracy, providing a fast, reliable, and cheap procedure to quantify phenolic metabolites in urine, and representing therefore a good and interesting alternative method. Also, the procedure offers other advantages, including the miniaturization, the low use of organic solvents, the ability to analyse small volumes of samples, in situ analysis and simple instrumentation requirement
Template and target information: 4-hydroxyphenylacetic acid, 4-HPA, flavan-3-ols
Author keywords: Molecularly imprinted polymer extraction, Polyphenol metabolites, Phenolic acids, Screen-printed carbon electrode, human urine, Adsorptive transfer differential pulse voltammetry