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Reference type: Journal
Authors: Herrera-Chacón A, Dinc-Zor S, del Valle M
Article Title: Integrating molecularly imprinted polymer beads in graphite-epoxy electrodes for the voltammetric biosensing of histamine in wines.
Publication date: 2020
Journal: Talanta
Volume: 208
Article Number: 120348.
DOI: 10.1016/j.talanta.2019.120348
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0039914019309816

Abstract: This manuscript presents a voltammetric biosensing study with use of molecularly imprinted polymers to detect histamine in wine. Polymer beads were synthesized by standard precipitation polymerization method and implemented on the electrode surface via sol-gel immobilization. Scanning and confocal microscopy examinations permitted characterizing the material. Adsorptive stripping voltammetry in differential mode was the technique chosen for final application, selecting an enrichment time of 5 min. These conditions permitted a limit of detection of 0.19 μg mL-1 (1.0 μM), with a linear response range from 0.5 to 6.0 μg mL-1 (2.71-32.4 μM). The repeatability of the measurements was 4.6% relative standard deviation (n = 12). Principal component analysis showed the ability of the prepared receptor for discriminating other biogenic amines and potential interfering species. A final application, illustrating the determination of histamine, was completed to show agreement of results between the fluorimetric reference method and the proposed electrochemical approach
Template and target information: histamine
Author keywords: molecularly imprinted polymer, sensors, Biogenic amines, histamine, voltammetry


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