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Reference type: Journal
Authors: Sun Z, Liu HC, Zhou YQ, Zhao SW, Li JM, Wang XX, Gong BL
Article Title: A restricted access molecularly imprinted polymer coating on metal-organic frameworks for solid-phase extraction of ofloxacin and enrofloxacin from bovine serum.
Publication date: 2019
Journal: RSC Advances
Volume: 9
Issue: (48)
Page numbers: 27953-27960.
DOI: 10.1039/C9RA04143E

Abstract: A restricted access molecularly imprinted polymer (RAMIP) crosslinked with bovine serum albumin (BSA) was prepared on the surface of the mesoporous UiO-66-NH2 metal-organic framework (MOF). The surface morphology, imprinting behavior, and protein exclusion properties of UiO-66-NH2@RAMIP@BSA were investigated. The maximum adsorption capacity was 50.55 mg g-1 for ofloxacin, with a 99.4% protein exclusion rate. Adsorption equilibrium was reached in 9 min. Combined with RP-HPLC, a solid-phase extraction column filled with UiO-66-NH2@RAMIP@BSA was used to selectively enrich and analyze ofloxacin and enrofloxacin antibiotics from bovine serum with recoveries of 93.7-104.2% with relative standard deviations of 2.0-4.5% (n = 3). The linear range and the limit of detection were 0.1-100 μg mL-1 and 15.6 ng mL-1, respectively. These results suggest that UiO-66-NH2@RAMIP@BSA is an efficient pretreatment adsorbent for biological sample analysis
Template and target information: ofloxacin, enrofloxacin

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