MIPdatabase
Authors: Zhang YD, Zhang Y, Ma C, Wang YY, Mu S, Liu XY, Zhang XY, Zhang HX
Article Title: Molecularly imprinted gelatin nanoparticles for DNA delivery and in-situ fluorescence imaging of telomerase activity.
Publication date: 2019
Journal: Microchimica Acta
Volume: 186
Issue: (9)
Article Number: 610.
DOI: 10.1007/s00604-019-3671-7
Abstract: DNA-loaded molecularly imprinted gelatin nanoparticles (GDMI-NPs) were prepared to deliver the Cy3- and Cy5-labelled DNA probe to a tumor region. This allows the activity of telomerase can be detected over 3-400 cells with a low detection limit (3 cells). Fluorescence images were acquired at an excitation wavelength of 535 nm and the emission from the green channel (550-580 nm; label Cy3) and the red channel (650-680 nm; label Cy5). HeLa cells and HepG2 cells were both used to test the performance of GDMI-NPs. Experimental results confirmed the GDMI-NPs has hardly retained in liver and spleen tissue, and its circulated time was longer than that of non-imprinted nanoparticles in blood. The ability of GDMI-NPs to resist immuno stress and anti-macrophage phagocytosis shows great potential for cancer diagnosis and as a drug carrier
Author keywords: transferrin, molecular imprinting, gene delivery, protein coronas, Immunological stress
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