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Abstract: An electrochemical sensor is described for determination of uric acid (UA). Carbon-enwrapped nickel nanoparticles (Ni@BC) were coated with polydopamine (PDA) that was molecularly imprinted with UA. The biomass carbon (BC) was synthesized by one-step solid-state pyrolysis from leaves of Firmiana platanifolia. The imprinted polymer was obtained by electrodeposition of DA as the monomer. The amount of monomer, the scan cycles, pH value and adsorption time were optimized. Furthermore, the selectivity of the MIP for UA on a glassy carbon electrode (GCE) was evaluated by selectivity tests. The differential pulse voltammetric responses to UA with and without interferents were consistent. The modified GCE has a linear response in the 0.01-30 μM UA concentration range, and the limit of detection is 8 nM. The MIP electrode was applied to the analysis of UA in urine for which the initial concentrations were determined by the phosphotungstic acid kit. Recoveries ranged from 91.3 to 113.4%, with relative standard deviations between 1.3 and 9.7% (n = 3)
Template and target information: uric acid, UA
Author keywords: Biomass carbon, Molecularly imprinted polymers, Electrochemical sensor, differential pulse voltammetry, urine