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Abstract: In this study, the attempt has been made in the direction to design a new artificial receptor biosensor by use of a hybrid method of molecularly imprinted polymer (MIP) and aptamers (Apt) to ultra-trace Trypsin (Trp) enzyme recognition Firstly, the effective surface of the electrode was increased by NiO nanoparticles electrochemical deposition Then a prepared complex containing Apt-Trp was incubated on the NiO surface A specific surface of Trp bind to one aptamer tail-end The other tail of the aptamer was attached to NiONP from the NH2 side and in this treatment, the protein target was immobilized as the template The electropolymerization process was performed using dopamine to completely form a polymeric matrix around the target protein Finally, the Trp molecule was eliminated with suitable eluent, and the prepared Apt-2DMIP biosensor was used for analytical experiments This real-time detective sensor had unparalleled selectivity and sensitivity in the presence of off-target species to the Trp A good diagnostic linear range from 1to 90 pg mL-1 was obtained with a detection limit of 0.75 pg mL-1 The imprinting factor was calculated without and in the presence of aptamer, and the results showed that aptamer significantly increased the throughput of the biosensor Finally, in real samples including blood human serum and urine samples, the efficiency of the biosensor was evaluated, and satisfactory results were obtained
Template and target information: protein, trypsin
Author keywords: Hybrid aptamer-MIP method, Trypsin biodevice, NiONP sub-layer, In-situ electropolymerization