Alternatively for mipdatabase quick searches go here

Custom Search

Abstract: In this work, the advantages of surface-enhanced Raman spectroscopy (SERS) sensitivity and molecularly imprinted technology selectivity were combined to achieve simultaneous detection of two biguanides, metformin hydrochloride (Met HCl) and phenformin hydrochloride (Phen HCl), in hypoglycemic health products. For this purpose, initially, gold nanoparticles (AuNPs) and graphene oxide (GO) were prepared. Subsequently, AuNPs were immobilized on GO through p-aminothiophenol to form the Au-GO composite. Finally, the molecularly imprinted polymer (MIP) was successfully prepared using Met HCl as the template molecule and coated onto the surface of the Au-GO composite to obtain the sandwich-structure substrate, MIP@Au-GO. Static and kinetic adsorption studies demonstrated the specific recognition of MIP@Au-GO for Met HCl and Phen HCl and a rapid adsorption equilibration rate. By simply incubating analytes with the MIP@Au-GO substrate, the proposed method showed strong SERS signal enhancement with a limit of detection of 0.10 mg/mL (S/N = 3) for both Met HCl and Phen HCl. The substrate also exhibited excellent reusability and long-term stability. Moreover, the method was successfully applied to the quantitative detection of Met HCl and Phen HCl in real hypoglycemic health products with satisfactory recoveries and standard deviations. The recoveries of Met HCl by MIP@Au-GO ranged from 87.5% to 112.5% with relative standard deviations (RSDs) of 0.8%-8.9%. The recoveries of Phen HCl ranged from 96.7% to 115% with RSDs of 3.1%-7.4%. The detection time is only 10 min. Overall, the novel approach appeared to be simple, rapid, and highly specific, thus could be effective for the on-site screening and detection of chemical drugs in complex matrices
Template and target information: metformin hydrochloride, Met HCl, phenformin hydrochloride, Phen HCl
Author keywords: Surface-enhanced Raman scattering, molecular imprinting, Antidiabetics biguanides, On-site detection, Health products