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Reference type: Journal
Authors: Sarabaegi M, Roushani M
Article Title: Rapid and sensitive determination of Pseudomonas aeruginosa by using a glassy carbon electrode modified with gold nanoparticles and aptamer-imprinted polydopamine.
Publication date: 2021
Journal: Microchemical Journal
Volume: 168
Article Number: 106388.
DOI: 10.1016/j.microc.2021.106388
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0026265X21004720

Abstract: Pseudomonas aeruginosa causes a long-term chronic disease that low concentrations of Pseudomonas aeruginosa can cause infection; therefore, rapid detection of this Pseudomonas aeruginosa is very important. This study presents a method with a combination of molecular imprinting and aptasensing in order to measure Pseudomonas aeruginosa (P. aeruginosa). The double exact molecular recognition property of the molecular imprinting polymers and aptamers led to superb sensing properties. In this study, gold nanoparticles were used as specific intermediate and interface in order to increase the aptamer sequence loading rate. The capability of this method was investigated by several electrochemical techniques. The sensor renders a wide linear dynamic concentration range of 10^1 to 10^7 CFU ml-1 with low detection limit of 1 CFU ml-1. In addition, satisfactory results were found in determining P. aeruginosa using blood samples. This sensor has many advantages, such as low cost, high stability, low response time, and high sensitivity for P. aeruginosa measurement
Template and target information: bacteria, Pseudomonas aeruginosa, P. aeruginosa
Author keywords: aptamer, AuNPs, P.aeruginosa, electropolymerization, molecular imprinting polymer


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