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Abstract: Potentiometric glutathione (GSH) sensors were fabricated using a molecularly imprinted polymer prepared from GSH, methacrylic acid (MAA), and ethylene glycol dimethacrylate as the template molecule, functional monomer, and cross-linker, respectively. Five GSH sensors were prepared with different ratios of GSH to MAA. Their potential responses were measured in a GSH aqueous solution using Ag/AgCl as the reference electrode. A GSH sensor prepared with a GSH:MAA ratio of 2:32 had the best responsivity, while the sensor synthesized from a non-imprinted polymer prepared without GSH (NIP sensor) showed a potential response value of almost zero after the addition of GSH. The ratio of the potential responses of the GSH sensor to the NIP sensor was 8.21. Additionally, the GSH sensor had good linearity over a GSH concentration range of 1 × 10-5 to 2 × 10-4 mol L-1. The GSH sensor provided good quantification and high specificity for GSH and is expected to be applicable for easy and direct determinations of GSH
Template and target information: glutathione, GSH
Author keywords: molecularly imprinted polymer, potentiometric sensor, Glutathione, quantification, specificity, methacrylic acid, functional monomer ratio, non-imprinted polymer, polymerization initiator, plasma polymerized membrane