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Abstract: Food manufacturers are aiming to manage the levels of cross-contamination of allergens within food processing plants and ultimately move away from precautionary labelling. Hence, the need for rapid methods to detect allergens cross-contamination. A sensitive and selective label-free nanoMIPs based sensor was developed and tested for the detection of β-lactoglobulin (BLG). NanoMIPs were synthesized using solid-phase synthesis and appeared as spherical nanoparticles with sizes ranging from 264-294 nm, using dynamic light scattering (DLS). The nanoMIPs were functionalized with amine groups and attached to the surface of the SPR gold chip via amine-coupling protocol. The SPR nanoMIPs-based sensor demonstrated a detection limit of 3 ng mL-1 (211 pM) over a linear range of 1-5000 ng mL-1, with binding affinity of 7.0 × 10-8 M and specificity towards BLG. With further testing and final optimization, the developed nanosensor can be integrated on-line or at-line cleaning-in-place (CIP) wash systems, allowing to effectively monitor milk protein allergens as a rapid, point-of-source methodology
Template and target information: protein, β-lactoglobulin, BLG
Author keywords: β-lactoglobulin (BLG), molecularly imprinted polymer (MIP), nanoparticles, surface plasmon resonance, food safety, allergen management