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Abstract: Extraction and purification of α- and β-amanitins from mushroom samples are still challenges. Surface-imprinted core-shell microspheres were synthesized combining target recognition determinant imprinting and thiol-ene click reaction, and applied to the extraction of α- and β-amanitins in mushroom samples. The highly efficient thiol-ene click reaction method offers a simple condition during the crosslinking process of molecularly imprinted polymers (MIP), and the functional monomer using could be successfully introduced onto the silica microspheres. The synthesized SiO2@MIP were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and thermogravimetric analysis (TGA). The measurement of SiO2@MIP resultants using high-performance liquid chromatography (HPLC) clearly demonstrates that SiO2@MIP not only display excellent affinity, but also show good selective recognition to α- and β-amanitins. The imprinted microspheres were successfully applied as sorbents for the extraction of α- and β-amanitins from mushrooms, attaining recoveries of 76.8-79.2%. Furthermore, the samples were effectively purified according to the chromatogram
Template and target information: epitope, amanitin, α-amanitin, β-amanitin
Author keywords: SPE (solid-phase extraction), Epitope and peptide separation, Thiol-ene click reaction, molecularly imprinted polymer, α- and β-amanitins, Selective sorbent