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Abstract: Unique restricted access media-magnetic molecularly imprinted polymers (RAM-MMIPs) were prepared as magnetic solid-phase extraction (MSPE) material for florfenicol (FF) and are combined with HPLC-UV to extract FF from fetal bovine serum samples. Under the optimization conditions, the resulting materials had a uniform imprinted layer, acceptable adsorption amounts (22.92 mg/g), rapid binding kinetics (80 min) and satisfactory selectivity. In addition, RAM-MMIPs presented satisfactory recoveries (95.6-102.1%), relative standard deviation (RSD) (2.7-3.9%) and lower limits of detection (LOD) (10.5 μg/L). The method was applied to the real samples. Compared with other reported methods, this method exhibits the advantages of simple, fast and, efficient operation, using a relatively small amount of the RAM-MMIPs adsorbent. The developed method can enrich FF from fetal bovine serum samples
Template and target information: florfenicol, FF
Author keywords: Florfenicol, Magnetic solid-phase extraction, Restricted access media-magnetic molecularly imprinted polymers, Fetal bovine serum, Specific recognition abilities, high performance liquid chromatography