MIPdatabase
Authors: Bezdekova J, Zemankova K, Hutarova J, Kociova S, Smerkova K, Adam V, Vaculovicova M
Article Title: Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus.
Publication date: 2020
Journal: Food Chemistry
Volume: 321
Article Number: 126673.
DOI: 10.1016/j.foodchem.2020.126673
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0308814620305355
Abstract: In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting. Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 × 10^3CFU ml-1, which corresponds to the limit set in European Union legislation for microbial control of food
Template and target information: bacteria, Staphylococcus aureus, S. aureus
Author keywords: Molecularly imprinted polymers, magnetic particles, dopamine, bacteria, fluorescence microscopy
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