MIPdatabase
Authors: Munawar H, Safaryan AHM, De Girolamo A, Garcia-Cruz A, Marote P, Karim K, Lippolis V, Pascale M, Piletsky SA
Article Title: Determination of Fumonisin B1 in maize using molecularly imprinted polymer nanoparticles-based assay.
Publication date: 2019
Journal: Food Chemistry
Volume: 298
Article Number: 125044.
DOI: 10.1016/j.foodchem.2019.125044
Alternative URL: https://www.sciencedirect.com/science/article/pii/S030881461931146X
Abstract: Fumonisin B1 (FB1) is a carcinogenic mycotoxin produced by Fusarium species contaminating maize. At present, fumonisin determination is performed using costly and demanding chromatography techniques or immunoassays. Recently, a molecularly imprinted polymer nanoparticles (nanoMIPs) - based assay (MINA) has been developed for FB1 detection. Herein, we have applied MINA for the determination of FB1 in naturally contaminated maize samples and results were compared with those obtained with ELISA and a reference HPLC method (AOAC No. 2001.04). The nanoMIPs as a recognition element mimicking antibodies used in ELISA were produced by solid phase synthesis and used in MINA for FB1 determination in 53 maize samples. As a result, 18 maize samples were contaminated with FB1 at levels higher than 0.25 mg/kg. Fumonisin concentrations from samples measured by MINA were well correlated with those using ELISA and HPLC. Therefore, MINA could be used as an alternative technique for FB1 determination in maize
Template and target information: fumonisin B1, FB1
Author keywords: Fumonisins, Maize, MINA, ELISA, HPLC
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