Alternatively for mipdatabase quick searches go here

Custom Search

Abstract: The use of a macromolecularly functional monomer and crosslinker (MFM) to stabilize and imprint a template protein is a new method to construct high-recognition protein-imprinted materials. In this study, for the first time, a "specially designed" block MFM with both "functional capability" and "crosslinking capability" segments was synthesized via reversible addition-fragmentation chain-transfer polymerization and used to fabricate bovine serum albumin (BSA)-imprinted microspheres (SiO2@MPS@MIPs-MFM) by the surface imprinting strategy. Results from circular dichroic spectrum experiments reflected that the block MFM could maintain the natural form of BSA, whereas its corresponding and equivalent micromolecularly functional monomer (MIM) seriously destroyed the secondary structure of proteins. Batch rebinding experiments showed that the maximum adsorption capacity and imprinting factor of SiO2@MPS@MIPs-MFM reached 314.9 mg g-1 and 4.02, which were significantly superior to that of MIM-based imprinted materials. In addition, since the crosslinking capability segments in block MFM involved zwitterionic functional groups with a protein-repelling effect, SiO2@MPS@MIPs-MFM showed better specific rebinding ability than the imprinted material prepared by MFM without this component. Besides, scanning electron microscopy and transmission electron microscopy images showed that the shell thickness of SiO2@MPS@MIPs-MFM was approximately 15 nm, and such a thin imprinted layer ensured its rapid adsorption equilibrium (120 min). As a result, SiO2@MPS@MIPs-MFM revealed fantastic selectivity and recognition ability in a mixed protein solution and could efficiently extract BSA from biological samples of bovine calf serum. The proposal of block MFM enriched the options and designability of monomers in protein imprinting technology, thereby laying a foundation for developing high-performance protein-imprinted materials
Template and target information: protein, bovine serum albumin, BSA
Author keywords: molecularly imprinted microsphere, protein recognition, functional monomer, Stability of protein, Zwitterionic polymer