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Conference information: Proceedings PACCON 2019

Abstract: Molecularly imprinted polymer (MIP) has become an outstanding selective element of clinically relevant analytes. In this study, we present a biomimetic surface imprinting strategy for human serum albumin (HSA), which can be found in both sera and urine. To begin with, the composition of the polymer was synthesized using the starting point of previously published MIP for bovine serum albumin (BSA). Both HSA and BSA have closely related properties in terms of size (14 × 4 × 4 nm) and heart-shaped molecule, but differ in the number of amino acids (585 amino acids of HSA, 583 amino acids of BSA), and also in types of amino acids (145 non-identical amino acids). HSA-MIP, comprising co-polymers of acrylic acid and N-vinylpyrrolidone at the ratio of 2:3 crosslinked with ethylene glycol dimethacrylate, were screened on 10 MHz dual-electrode quartz crystal microbalances (QCM) for binding ability investigation. Such MIP yields much higher sensor response towards 3 mg/mL of HSA concentration (520 Hz) than other urinary substances at ten-time higher physical relevant concentrations (73 Hz of 0.13 mg/mL creatinine, 0 Hz of 2.5 mg/mL cortisol). It indicates that MIP favors HSA very strongly and can be used further to develop device for albumin determination in urine.
Template and target information: protein, human serum albumin, HSA