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Abstract: Exosomes (30-150 nm) secreted by cells play an important role in intercellular communication. Herein, a novel double imprinting-based electrochemical method has been developed for analyzing the particle size distribution (PSD) of mimetic exosomes - a mixture of SiO2@HRP with the diameters in 50 nm, 100 nm and 150 nm at a ratio similar to the exosomes. Double imprinting polymers (DIP) were synthesized with SiO2@HRP at different sizes as template, providing the both recognition of size and morphology. With the capture of the target and the binding of the signal amplification tag - SiO2@Ag/MPBA on the DIP film, a sandwich structure was constructed on Au NPs-GO modified glassy carbon electrode. Under the optimal conditions, the DIP film exhibits the linear correlations between the current intensity and the logarithmic concentration of SiO2@HRP as Δ I50 = -1.52 + 0.50 x lgc50 (2.89 × 10^4 - 2.89 × 10^9 particles/mL), Δ I100 = -1.61 + 0.48 x lgc100 (2.89 × 10^4 - 2.89 × 10^9 particles/mL) and Δ I150 = -1.54 + 0.49 x lgc150 (5.75 × 10^4 - 5.75 × 10^9 particles/mL) with the limit of detection of 1.44 × 10^3 particles/mL, 5.68 × 10^2 particles/mL and 7.70 × 10^2 particles/mL, respectively. Furthermore, the DIP film was applied to the PSD analysis of mimetic exosomes - SiO2@HRP at mixed sizes with a ratio of 50 nm:100 nm:150 nm = 5.0%:42.5%:52.5% detected by nanoparticle tracking analysis (NTA), obtaining a similar result of 50 nm:100 nm:150 nm = 3.6%:43.0%:53.4% with small relative concentration errors. It also exhibited excellent reproducibility and stability. This double imprinting-based method shows high potential in the separation and detection of complex biosamples
Template and target information: exosome mimics, SiO2@HRP, nanoparticles
Author keywords: Double-imprinting, Particle size distribution, Mimetic exosomes, electrochemical sensing