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Abstract: Herein, we present and discuss the development of a novel sensor for aflatoxin B2 (AFB2) determination by two methods of functionalization. The first method was performed, by the electrodeposition of zinc oxide nanoparticles (ZnO-NPs), coated with a mixture of chitosan (CS) and AFB2 on the gold-working area of a screen-printed electrode. As for the second functionalization method, it was carried out by electropolymerization of pyrrole (Py) and AFB2 to form polypyrrole/AFB2 (PPy/AFB2) on another screen-printed electrode already modified with ZnO-NPs/CS composite. The morphology and the chemical composition of functionalized electrode surfaces were studied by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FT-IR). This system was successfully applied to determine AFB2 residues in fresh and pasteurized milk, with an excellent selectivity when compared to the results of interfering molecules. The limits of detection (LOD) and quantification (LOQ) were found to be 0.6 and 2.0 fg mL-1 for ZnO-NPs/CS/AFB2 functionalized hybrid composite, and 0.2 and 0.7 fg mL-1 for ZnO-NPs/CS/PPy/AFB2 functionalized hybrid composite when DPV measurements were used, respectively. The proposed technique is cheap, sensitive, selective, and is applied successfully to detect unmetabolized AFB2 in milk samples. Considering these advantages, our system provides an emerging approach application in industrial food control
Template and target information: aflatoxin B2, AFB2
Author keywords: Aflatoxin B2 detection, ZnO nanoparticles, chitosan, Molecularly imprinted sensor, milk, Food control