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Abstract: Graphitic carbon nitride (g-C3N4), as a visible-light-active organic semiconductor, has attracted growing attentions in photocatalysis and photoluminescence-based biosensing. Here, we demonstrated the intrinsic photooxidase activity of g-C3N4 and then surface molecular imprinting on g-C3N4 nanozymes was achieved for improved biosensing. Upon blue LED irradiation, the g-C3N4 exhibited superior enzymatic activity for oxidation of chromogenic substrate like 3,3',5,5'-tetramethylbenzidine (TMB) without destructive H2O2. The oxidation was mainly ascribed to O2- that was generated during light irradiation. The surface molecular imprinting on g-C3N4 can lead to an over 1000-fold alleviation in matrix-interference from serum samples, 4-fold improved enzymatic activity as well as enhanced substrate specificity comparing with bare g-C3N4 during colorimetric sensing. Also, the MIP-g-C3N4 possesses a high affinity to TMB with a Km value of only 22 μmol/L, much lower than other comment nanozymes like AuNPs, Fe3O4 NPs, etc. It was successfully applied for detection of cysteine in serum sample with satisfactory recoveries
Template and target information: 3,3',5,5'-tetramethylbenzidine, TMB
Author keywords: molecularly imprinted polymer, Graphitic carbon nitride, Nanoenzyme, Colorimetry, Biosensing