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Abstract: As a by-product of Chinese olive, an important economic tree, Chinese olive leaves have a large amount of hydroxytyrosol and other ingredients with high biological activity. At present, Chinese olive leaves can only be treated as waste and animal feed, causing a lot of waste of resources and pollution to the environment. Magnetic molecularly imprinted polymers using hydroxytyrosol as a template molecule were synthesized by surface imprinting molecular technology. Several ways, such as X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscope and vibrating sample magnetometer, were used to perform physicochemical characterization of Fe3O4@MIPs. The results of the kinetic and static adsorption experiments showed that adsorption equilibrium could be reached within 45 min, with a maximum adsorption capacity of 36.27 mg g-1. Fe3O4@MIPs had good selectivity and specificity for hydroxytyrosol. Under the optimum conditions, an efficient method based upon MISPE combined with high-performance liquid chromatography (HPLC) was developed for the determination of hydroxytyrosol in Chinese olive leaves with the high recovery rate (96.71-98.06%) and relative standard deviations of 2.35-3.27%. Therefore, this research provided a new method for the separation, purification and determination of hydroxytyrosol from Chinese olive leaves, improved the resource utilization of Chinese olive leaves, and protected the environment
Template and target information: hydroxytyrosol
Author keywords: Magnetic molecularly imprinted polymers, Hydroxytyrosol, Solid-phase extraction, molecular recognition, Chinese Olive leaves