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Abstract: The residues of pyrethroids in foods of animal origin are dangerous to the consumers, so this study presented a chemiluminescence sensor for determination of pyrethroids in chicken samples. A dual-dummy-template molecularly imprinted polymer capable of recognizing 10 pyrethroids was synthesized. The results of computation simulation showed that the specific 3D conformations of the templates had important influences on the polymer' recognition ability. The polymer was used to prepare a sensor on conventional 96-well microplates, and the sample solution was added into the wells for direct absorption. The absorbed analytes were initiated with the bis(2,4,6-trichlorophenyl)oxalate-H2O2-imidazole system, and the chemiluminescence intensity was used for analyte quantification. Results showed that one assay was finished within 12-ámin, and this sensor could be reused four times. The limits of detection for the 10 analytes were in the range o0.3-6.0-ápg/ml, and the recoveries from the standards of fortified blank chicken samples were in the range 70.5-99.7%
Template and target information: dummy template, dual template, pyrethroids
Author keywords: Chicken, Computation simulation, Dual-dummy-template, microtitre chemiluminescence sensor, molecularly imprinted polymer, pyrethroids