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Reference type: Journal
Authors: Dickert FL, Hayden O, Bindeus R, Mann KJ, Blaas D, Waigmann E
Article Title: Bioimprinted QCM sensors for virus detection - screening of plant sap.
Publication date: 2004
Journal: Analytical and Bioanalytical Chemistry
Volume: 378
Issue: (8)
Page numbers: 1929-1934.
DOI: 10.1007/s00216-004-2521-5

Abstract: Surface imprinting techniques on polymer-coated quartz-crystal microbalances (QCM) have been used to detect tobacco mosaic viruses (TMV) in aqueous media. Molecularly imprinted polymers (MIP), tailor-made by self organisation of monomers around a template (TMV), were generated directly on the gold electrodes. Imprinted trenches on the polymer surface mimicking the shape and surface functionality of the virus serve as recognition sites for re-adsorption after washing out of the template. The sensors are applicable to TMV detection ranging from 100 ng mL(-1) to 1 mg mL(-1) within minutes. Furthermore, direct measurements without time-consuming sample preparation are possible in complex matrices such as tobacco plant sap


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