MIPdatabase
Authors: Lehmann M, Dettling M, Brunner H, Tovar GEM
Article Title: Affinity parameters of amino acid derivative binding to molecularly imprinted nanospheres consisting of poly[(ethylene glycol dimethacrylate)-co-(methacrylic acid)].
Publication date: 2004
Journal: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume: 808
Issue: (1)
Page numbers: 43-50.
DOI: 10.1016/j.jchromb.2004.03.068
Alternative URL: http://www.rci.rutgers.edu/~moghe/Lecture14B_MS.pdf
Abstract: The binding of -Boc-phenylalanine anilide (BFA) and -Boc-phenylalanine (phe) to molecularly imprinted and non-imprinted polymer nanoparticles consisting of poly[(ethylene glycol dimethacrylate)-co-(methacrylic acid)] has been investigated by adsorption experiments and mathematical modeling. The experimental isotherms have been mathematically adapted following the models of Freundlich, Langmuir, Langmuir-Freundlich, Bi-Langmuir, and extended Langmuir. The extended Langmuir model differentiated between specific and nonspecific binding of the ligand to the receptor nanoparticles and rendered excellent fitting of the experimental data. It delivered a thermodynamic and kinetic parameter set on the experimental association curves of -BFA by -BFA-imprinted nanospheres in suspension experiments with the equilibrium constant KD=4.09+/-0.69 μmol L-1 and the kinetic association rate constant ka=5.60 mL μmol-1 min-1
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